SIGHTINGS



Cloning Of Frozen
Mammoths Remains Elusive
By Beverly Jaegers
12-1-99
 

 
Are these hairy giants once again to share the Planet with man? "A mammoth in every Zoo?"
 
Will cloning techniques provide a closer look at a long-vanished behemoth?
 
Only in the last decade has a serious attempt been made at restoring the mammoth to life once more. Unlike Jurassic Park and its fictional attempt at repopulating extinct dinosaurs via the cloning lab, today's technological successes have resulted in methods that may indeed restore a less fearsome yet equally fascinating animal - not dead 65 to 200 million years but one that actually lived amongst men only four or five thousand years ago.
 
Different techniques will be applied to this effort than the art- ificial egg sequence. What will be attempted will be the insertion of extinct DNA into the embryo of a living cousin of the woolly mammoth - the asian elephant, a close cousin of the earlier herbivore.
 
FROZEN TREASURE
 
An entire frozen mammoth was first discovered on the shores of the Arctic ocean by a native Tungus Chief named Ossip Shamakov, who spotted something large and dark inside a huge block of ice along a riverbank where he'd been searching for ivory. The year was 1799, and the last vestiges of a hot August had melted away enough ice to allow Shamakov to see the dim outline of a very large animal under the glassy surface. The lateness of the season did not allow for any further investi- gation at that time, and when Shamakov returned the following summer he could see the animal much more clearly, yet it was still unreachable.
 
July 1801 saw him returning to the banks of the Lena delta creek where he could now see a single tusk and a hairy shoul- der protruding from the ice. Being a superstitious man, he remembered a tribal prohibition against touching such animals and hurried away in fear to collect the scattered tusks whose owners had safely become anonymous skeletons millenia before. He was certain he'd not come close enough to get the mysterious sickness touching a mammoth could cause.
 
He did become sick that autumn, but the sickness ended and he regained his health and his ambition to go back to the Lena delta. Mammoth ivory was highly prized in those times, as it is now, and the promise of 25 rubles for each tusk kept him from forgetting what he'd seen.
 
Two summers later, he returned to the Siberian delta area to see if the elephant-like animal were still there. It was. He eventually helped remove the tusks with the help of an ivory dealer, who made a crude sketch of it, which was then shown to a scientist.
 
In 1806 the mammoth had partially been exposed on its right side and naturally eaten by wolves and other predators. The left side was still embedded in solid ice and in good condition. Ten workmen hired by the scientist spent weeks carving it free and shipping the remains to St. Petersburg in Russia, where it brought the princely sum of 8,000 rubles. This skeleton was mounted and is still found in the St. Peters- burg Museum.
 
MAMMOTH OR MASTODON?
 
It was to be many decades before another such find was made, although the bones and fossils of the once-furry animals which so resembled the contemporary tusked elephant were to be found scattered in areas as far flung as Mexico and Ohio, and were recognized as early as 1519 on the American continents.
 
Mammoth and mastodon bones were considered to have belonged to the same animals now considered to have become extinct by scientists bold enough to believe one of 'God's creations' had been allowed to vanish from the Earth. There were differences, however, mainly in the teeth of some fossils, which fascinated such American luminaries as Benjamin Frank- lin and Thomas Jefferson, whose hobbies included what we now term Paleontology.
 
These differences in fossil teeth are the distinguishing factor between mammoth and mastodon, for whereas mammoth teeth all had raised parallel ridges in regular placement on their grinding molars; some teeth found in America and Europe had nine lumpy knobs on the same teeth. This difference led to the animals being classified as mastodons if the teeth possessed these lumps, and considered not to be related to modern ele- phants at all. The name 'mastodon' was coined in 1814 for the lumpy-toothed carcasses.
 
Both animals had roamed the continents far into the ranges of the Arctic at apparently the same times...but the differences still pose a small mystery to the scientist. (to the end of his life, Jefferson insisted that they were all mammoths)
 
These hairy herbivores had ranged the cold, dry areas near most of the Northern portions of north America, Europe and Asia both during and between glaciations. Areas near huge ice-age glaciers were not wet and snowy as is thought, but are often dry and bitterly cold, as glaciers by their very nature attract much of the available moisture in the atmosphere to their own surfaces.
 
Creatures with dense fur such as the small-eared mammoth and mastodon, accompanied by enormous cave-dwelling bears, furred rhinoceros-cousins, wolves and long-toothed cave lions, which are the reality behind stories of sabretoothed tigers, giant deer and steppe horses were well fitted for intense cold and ranged the dry, frigid plains in huge numbers. In death, bones of these creatures were often carried into huge piles by glacial runoff in the spring months, thus creating treasure troves of sturdy building materials for early man dwel- ling in these inhospital climes, plus a seemingly endless supply of ivory teeth and tusks for decorative purposes. Many such sites exhibit burned bone in fireplace rings, although it takes a very creative method of firebuilding to burn bone efficiently.
 
Later, these immense bonefields puzzled explorers and intri- gued fossil hunters. A number of these piled and tumbled bonebeds turned up at the base of cliffs and were ascribed to natural stampeding by wildfire or other sources; until the for- tuitous discovery of worked flint points in close proximity to the carcass piles. Once a number of these points had been recovered from between or even embedded into mammoth bones there could be no other conclusion than that they'd been stampeded by man. Some sites included distinct signs of butchering and dismembering of the huge, shaggy animals.
 
Another hint of the real predator, man, is that the very name 'mammoth' is derived from 'mammut' or 'mountain of meat' which is current in some steppes tribes. The hypothesis of man existing in these long gone ages was further cemented in more recent times by the discovery of carved representations of the tusked mammoth deeply incised into ivory from the animal itself - thus proving that the ivory had not been recovered from fossil beds, but from living animals.
 
This discovery expunged the idea that giant humans had walked the earth in prediluvian times, and began the process of establishing the study of ancient beginnings for both animal and man as contemporaries in a world far removed in climate as well as time. In addition they pushed man's understanding of true antiquity for both back many millenia beyond the supposed four to five thousand-year record of the Biblical scholar. The old ideas died hard, but in the face of evidence it was understood by most that both animals and human had existed for immensely longer periods of time than had been thought.
 
Glacial moraines and debris existed across the world, and were easily discovered in even cursory studies of Europe from the polar region to the southern Alps in obvious layered depos- its, the uppermost of which were found to have receded a scant 11,000 years ago. Older layers boasted datings of as much as 225,000 years previous to the most recent.
 
These mile-thick ice sheets came and went in successive periods, each providing optimum climatic conditions for huge herds of furry creatures adapted to the cold, while the milder eras encouraged their migration northwards for a time. In between these periods, more temperate and tropical conditions encouraged the proliferation of non-furred animals who crowd- ed into balmier areas of river, forest and plain. When these were once again covered with ice, the huge migrations began again, each leaving its evidence in fossil beds.
 
It was in one of the epochs known to be close to glacial that a jawbone and a group of worked tools was located, thus prov- ing that man had populated Europe in the warmer period after the first ice age, when mammoths still remained in the south.
 
When the third glaciation occurred, 230,000 years ago, humans seem to have discovered adaptive ways to stay and hunt the abundant supply of meat roaming just beneath the towering glacial sheets or at the edge of smaller mountain glaciers which dotted the land in lower latitudes. An ice-free zone extended from modern France eastward and plentiful evidence exists here of mammoth-hunting man living in rock shelters under deep overhangs where he hunted and left decorated mammoth ivory carvings and representations of many other animals they hunted in the treeless tundra.
 
The fourth such icy surge saw in its retreat more modern tools and artifacts crowding on its heels, proving that direct ances- tors of contemporary humanity established strong beachheads, an ever-expanding habitat which extended slowly to north and south, following the herds of woolly prey.
 
During this time of upheaval and repeated migration, mammoth and other sub-arctic animals had nourished their human con- temporaries with meat, hides, water-holding bladders and an easily usable store of arching tusk and sturdy bone with which to create foundations and walls of warm earth-covered lodges sheltering their occupants from the cold in a treeless land.
 
Man, once again, had demonstrated his innate ability to adapt and thrive in severest weather and the most inhospitable of climatic conditions.
 
FOREST ELEPHANTS
 
Like an earlier dominant species, the dinosaur, heavy-furred mammoths seemed selected for extinction after the last retreat of the heavy ice.
 
Remnants, however, existed until the pyramids were under construction, although totally isolated on a sub-arctic island and unseen by man. 'Forest elephants' had long been reported in some areas into the last of the 19th century, though contem- porary science pooh-poohed the idea due to lack of evidence and the unsophisticated natives who provided such sightings. It is the very lack of sophistication that lends credibility to these reports, as natives with no formal education are hardly likely to have knowledge of long-extinct species, nor their rarity and value to a researcher. So we may have missed the experience of living specimens by less than a century.
 
Unlike the coelacanth, long thought extinct until a living spec- imen was caught, mammoths are unlikely to be found roaming the landscape undiscovered by modern satellite surveillance of the planet.
 
FLYING MAMMOTH
 
In 1999, a field paleontologist recovered what was, for him, the 'holy grail' - a frozen male mammoth, in good enough condition to completely study, then attempt to clone. This had been his goal since 1978 or 79.
 
Larry Agenbrod joined an expedition led by Bernard Buigues of France, partially funded by Discovery Channel, with the in- tention of finding an adult bull mammoth in the condition necessary to provide answers to a vast number of questions left unanswered by fossil record.
 
For decades, such carcasses, appearing quick-frozen, have turned up with often a sample of its regular diet of plants em- bedded in its frozen jaws or in the ice near the body. Most were lost through lack of technological expertise or equipment capable of preserving them.
 
It became Agenbrod's obsession to join a veteran explorer like Buigues, capable of leading such a venture. The idea also required a source of funding sufficient to not only locate, but to re-locate a male mammoth to some location where it could be slowly thawed and completely studied.
 
With Dutch paleontologist Dick Mol, Agenbrod set up an am- bitious plan of attack. Siberian cooperation was secured, and a likely hunt area found.
 
Shortly after the plans came together, a family of reindeer hunt- ers contacted authorities with news of a find. In mid 1997, onsite confirmation was made and recovery methods outlined.
 
RESCUING JARKOV
 
The massive giant, more than 11 feet at his woolly shoulders, weighed six tons and had been icelocked for an estimated 20,000 years, since before the last glacial advance. The big bull, named Jarkov for the reindeer-hunters who found him, lay deep in the ice. A convenient icelab location lay just above him and a refrigerated building was built over his frozen body at the dig site.
 
When excavated in an iceblock, a team flew him 150 miles to Katanga, where an ice cave dug by Stalin's gangs of convict laborers had proven to be the perfect lab location.
 
Thawing Jarkov is being done the modern way, with hair dryers gently blowing the thick fur and 2 to 3 foot long guard hairs aside to reveal intact skin, muscle and bone.
 
Jarkov, unlike the smaller mammoths found in the island location where they'd evolved into a dwarfed condition to survive, is a full-sized mammoth which should provide the optimum best chance at cloning.
 
Jarkov's body will yield more than just DNA, however, as his condition is excellent, and exploration of his well-preserved body should help provide answers long sought which include why the mammoth became extinct at all. Virus and bacteria information as well as pollen or vegetation samples may be located in the body or the fur which may provide invaluable information about the prehistoric world.
 
TO LIVE ONCE MORE
 
Twenty-thousand year old DNA will prove a stiffer challenge than that of contemporary sheep or human genetic material, but insiders feel the risky process has a good chance of suc- cess. The world's best will be recruited to work with Jarkov's sperm. If successful, an asian elephant female will be the receptor of the previous, ancient DNA.
 
Successful cloning could be the result desired by all of the recovery team, even if this is not the single goal.
 
Jarkov, whose earlier life ended at the fairly young age of 47, may help combine the double helix of both ancient life and modern life to provide researchers with a chance to restore what would otherwise have been impossible - a living denizen of a long-ago prehistoric world.
 
No matter what the result of this amazing science coopera- tion, the boundaries of the possible will have been pushed to the edge of the envelope once more - and beyond.
 
_____
 
The author, Beverly Jaegers, is leader of the U. S. Psi Squad, and a journalist whose work has been published in magazines and books since 1968.
 
Her interest in science is reflected both in the Police-assistance group she organized, and in the resource work done for scientists in the fields of archaeology and pre-history.
 
The Psi Squad's website is www.alltel.net/~computir/uspsisquad/ (computir is the correct spelling - please note) Email is USPsiSquad@aol.com


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