Share Our Stories! - Click Here

From Dr. Betty Martini, D.Hum
6-6-17

Oxidative stress evoked damages on rat sperm and attenuated antioxidant status on consumption of aspartame

Ashok I¹, Poornima PS², Wankhar D¹, Ravindran R¹, Sheeladevi R¹.

Author information


Abstract

Although several studies on toxic effect of aspartame metabolite have been studied, controversial reports over the use of aspartame owing to the fact that it releases methanol as one of its metabolite during metabolism exist. This present study is proposed to investigate whether aspartame (40 mg kg^-1 b.wt) administration for 90 days could induce oxidative stress and alter antioxidant status of epididymal sperm in Wistar strain male albino rats. To mimic the human methanol metabolism, methotrexate (MTX)-treated rats were included to study the effects of aspartame. Oral intubations of FDA approved 40 mg kg^-1 b.wt aspartame were given daily for 90 days to Wistar strain male albino rats and studied along with controls and MTX-treated controls. Sperm count, viability, morphology, morphometry and motility were assessed. A significant decrease in sperm function of aspartame treated animals was observed when compared with the control and MTX control. The free radical generation were observed in epididymal sperm by assessing the scavenging enzymes, enzymatic and non-enzymatic antioxidants. Result suggest that there was a significant increase glutathione-s-transferase (GST), with a significant decrease in reduced glutathione (GSH), superoxide dismutase activity (SOD), glutathione peroxidase levels (GPx), catalase activity (CAT) and glutathione reductase concentration. The increase in free radicals generation could have ultimately caused the lipid peroxidation mediated damages on the testis. Aspartame treated animals also revealed the reduced space in seminiferous tubules, which resulted in reduced Leydig cells when compared with control in histopathology. These findings demonstrate that aspartame metabolites could be a contributing factor for development of oxidative stress in the epididymal sperm.International Journal of Impotence Research advance online publication, 27 April 2017; doi:10.1038/ijir.2017.17.PMID:

28446800

DOI:

10.1038/ijir.2017.17

• Share on Facebook

• Share on Twitter

• Share on Google+

LinkOut - more resources

PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

Save items

Add to FavoritesView more options

Similar articles

•  Effect of chronic exposure to aspartame on oxidative stress in the brain of albino rats.[J Biosci. 2012]

• Biochemical responses and mitochondrial mediated activation of apoptosis on long-term effect of aspartame in rat brain.[Redox Biol. 2014]

• Acute effect of aspartame-induced oxidative stress in Wistar albino rat brain.[J Biomed Res. 2015]

• Improvement of mercuric chloride-induced testis injuries and sperm quality deteriorations by Spirulina platensis in rats.[PLoS One. 2013]

• Quercetin attenuates lambda cyhalothrin-induced reproductive toxicity in male rats.[Environ Toxicol. 2013]

See reviews... See all...

More information on aspartame on www.mpwhi.com, www,holisticmed.com/aspartame, www.aspartamekills.com

Dr. Betty Martin, D.Hum, Founder
Mission Possible World Health Intl