- From: Alan Cantwell
- Date: February 23, 2008 2:46:58 PM PST
- To: Greg Blaney
- Cc: Trevor Marshall
- Subject: Re: "Acne bacteria" (P.acnes) repeatedly
found in prostate cancer!! More proof of "cancer bacteria"!!
- To Greg Blaney, MD:
- Thanks for notifying me regarding this important paper
published in the Journal of Clinical Microbiology in Nov, 2007......more
proof that "cancer bacteria" are involved in cancer -- and
particularly in prostate cancer -- now if only we could get pathologists
to see these bacteria in acid-fast stained sections of prostate ca --
as noted by me in my partner's tissue sections taken from his prostate
cancer when his prostate gland was removed in 2004 - and pictured below
in the paper published in the Journal of Independent Medical Research
(www.joimr.org) in 2004.
- As you note -- from abstracts you attached below, this
is NOT the first time Propioniobacterium acnes has been associated with
prostate cancer......it amazes me that this research is largely ignored
by urologists (and also by the media) -- like Helicobacter pyloris in
stomach ulcers was ignored for decades by physicians.
- With prostate cancer being an older man's worst nightmare
-- one would expect greater interest in this!
- Best regards,
- ALAN CANTWELL, MD
- On Feb 23, 2008, at 9:10 AM, Greg Blaney wrote:
- 1: J Clin Microbiol. 2007 Nov;45(11):3721-8. Epub 2007
Sep 19. Links
- Direct visualization of Propionibacterium acnes in prostate
tissue by multicolor fluorescent in situ hybridization assay.
- Alexeyev OA, Marklund I, Shannon B, Golovleva I, Olsson
J, Andersson C, Eriksson I, Cohen R, Elgh F.
- Department of Pathology, Umeå University, S-90185,
Umeå, Sweden. email@example.com
- Prostate tissues from patients with prostate cancer and
benign prostatic hyperplasia (BPH) frequently contain histological inflammation,
and a proportion of these patients show evidence of Propionibacterium
acnes infection in the prostate gland. We developed a multicolor fluorescent
in situ hybridization (FISH) assay targeting P. acnes 23S rRNA along with
a 14-kb region of the P. acnes genome. This assay was used to analyze
prostate tissues from patients with prostate cancer and BPH. P. acnes
infection of the prostate gland was demonstrated in prostatic tissue in
5 of 10 randomly selected prostate cancer patients.
- FISH analysis and confocal laser microscopy imaging revealed
intracellular localization and stromal biofilm-like aggregates as common
forms of P. acnes infection in prostate tissues from both prostate cancer
and BPH patients. A sequential analysis of prostate tissue from individual
patients suggested that P. acnes can persist for up to 6 years in the
prostate gland. These results indicate that P. acnes can establish a persistent
infection in the prostate gland. Further study is needed to clarify the
link between this bacterium and prostatic inflammation which may contribute
to the development of BPH and prostate cancer.
- PMID: 17881550 [PubMed - indexed for MEDLINE]
- Acid-Fast Bacteria In-Vivo in Prostate Cancer and the
Connection between Prostate Cancer, Other Cancers, and the Kaposi's Sarcoma
- Author: Alan R Cantwell, Jr., M.D.
- Los Angeles, California,
- contact email: alancantwell@sbc global.net
- Paper Type: Review
- Please cite as: Cantwell AR Jr. Acid-Fast Bacteria In-Vivo
in Prostate Cancer and the Connection between Prostate Cancer, Other
Cancers, and the Kaposi's Sarcoma Virus. JOIMR 2004;2(3):1
- Published: 28 May 2004
- (C) 2004, by Alan Cantwell, Jr. M.D.
- Bacteria in the form of previously described "cancer
microbes" were identified in a Fite (acid-fast)-stained histopathologic
tissue section from a case of prostate adenocarcinoma in a 68 year- old,
HIV-negative Italian-American man with a concomitant history of a solitary
skin lesion of classic Kaposi's sarcoma. The pleomorphic bacteria observed
in vivo are consistent with so-called cancer-associated bacteria previously
described in breast cancer, Kaposi's sarcoma, and other forms of cancer,
by various investigators over the past century. The proposed microbiology
of cancer is discussed, as well as the new finding of the Kaposi's sarcoma
virus (human herpes virus-8) in the blood of up to 40% of patients with
prostate cancer. Further studies of acid-fast bacteria in vivo and the
KS virus in prostate cancer are sorely needed to further determine the
possible role of these infectious agents in this common cancer of men.
- 1: J Urol. 2000 Oct;164(4):1221-8.
- Related Articles, Links
- Bacterial dna sequences in prostate tissue from patients
with prostate cancer and chronic prostatitis.
- Krieger JN, Riley DE, Vesella RL, Miner DC, Ross SO,
- Department of Urology, University of Washington, School
of Medicine, Seattle, Washington, USA.
- PURPOSE: Although bacterial genetic material has been
detected in prostate tissue from patients with various disorders, the
prevalence of these organisms is unknown. We tested the hypothesis that
bacterial detection rates differ between patients with prostate cancer
and those with the chronic prostatitis/pelvic pain syndrome. MATERIALS
AND METHODS: Sterile prostate biopsies were obtained during radical retropubic
prostatectomy from 107 patients with prostate cancer and using a perineal
approach from 170 with the chronic prostatitis/pelvic pain syndrome. Numerous
controls were also evaluated. Bacterial ribosomal encoding DNA (165 rDNA)
sequences were detected using a polymerase chain reaction assay. Selected
positives were cloned, sequenced and compared with DNA databases. RESULTS:
Bacterial DNA sequences were detected in 21 (19. 6%) of 107 patients with
prostate cancer compared to 79 (46.4%) of 170 with chronic prostatitis
(p <0.0001). These bacteria included urogenital pathogens, other described
microorganisms and bacteria not reported previously. CONCLUSIONS: Bacterial
DNA sequences may be identified in prostate tissue from many patients.
Bacterial detection rates in prostate tissue appear to differ among populations,
with higher rates among patients with the chronic prostatitis/pelvic pain
syndrome than among those with prostate cancer. Future studies of the
role of various bacteria in the prostate may provide insight into the
pathophysiology of prostate disease.
- 1: J Urol. 2005 Jun;173(6):1969-74.
- Related Articles, Links
- Propionibacterium acnes associated with inflammation
in radical prostatectomy specimens: a possible link to cancer evolution?
- Cohen RJ, Shannon BA, McNeal JE, Shannon T, Garrett KL.
- Uropath Pty Ltd, Perth, Western Australia. firstname.lastname@example.org
- PURPOSE: Inflammation is commonly observed in the prostate
gland and has been implicated in the development of prostate cancer. The
etiology of prostatic inflammation is unknown. However, the involvement
of a carcinogenic infectious agent has been suggested. MATERIALS AND METHODS:
Prostatic tissue from 34 consecutive patients with prostate cancer was
cultured to detect the presence of bacterial agents. Prostatic inflammation
was assessed by histological examination of wholemount tissue sections.
RESULTS: The predominant microorganism detected was Propionibacterium
acnes, found in 35% of prostate samples. A significantly higher degree
of prostatic inflammation was observed in cases culture positive for
P. acnes (p =0.007). P. acnes was separated into 3 groups based on cell
surface properties, phenotype and genetic grouping. All skin control isolates
were classified as group 1 whereas most prostatic isolates were classified
as groups 2 and 3. CONCLUSIONS: P. acnes has been isolated from prostatic
tissues in men who underwent radical prostatectomy for localized cancer
and has been shown to be positively associated with prostatic inflammation.
This inflammation may then be linked to the evolution of carcinoma. Furthermore,
organisms infecting these patients with prostate cancer differ genetically
and phenotypically from the commonly identified cutaneous P. acnes isolates,
suggesting that specific subtypes may be involved in development of prostatic
- 1: Future Oncol. 2006 Apr;2(2):225-32. Links
- Links between Propionibacterium acnes and prostate cancer.
- Shannon BA, Garrett KL, Cohen RJ.
- Tissugen Pty Ltd, Perth, Western Australia.
- Incidental foci of prostate cancer are found at autopsy
in 30% of men in their third decade, and by their eighth decade more than
75% have histological evidence of cancer. This unprecedented cancer prevalence
points to a ubiquitous causative agent or perhaps an interaction between
multiple common carcinogenic cofactors. We propose that one of these carcinogens
is Propionibacterium acnes. Several characteristics of prostate cancer
suggest the involvement of an infectious agent and we provide evidence
that P. acnes is an excellent candidate. We have cultured P. acnes from
a substantial proportion of prostate glands containing cancer and shown
a significant positive association with prostatic inflammation. P. acnes
is well suited to cause persistent, low-grade infection involving a marked
inflammatory response and the P. acnes subtypes most frequently associated
with prostate cancer become highly prevalent in the urinary tract of males
- PMID: 16563091 [PubMed - indexed for MEDLINE]
- 1: Res Microbiol. 2007 May;158(4):386-92. Epub 2007 Feb
- Biofilm formation by Propionibacterium acnes is associated
with increased resistance to antimicrobial agents and increased production
of putative virulence factors.
- Coenye T, Peeters E, Nelis HJ.
- Laboratorium voor Farmaceutische Microbiologie, Universiteit
Gent, Harelbekestraat 72, B-9000 Gent, Belgium. email@example.com
- Propionibacterium acnes plays an important role in the
pathogenesis of acne vulgaris, a common disorder of the pilosebaceous
follicles. Recently, it was suggested that P. acnes cells residing within
the follicles grow as a biofilm. In the present study, we tested the
biofilm-forming ability of several P. acnes strains in a microtiter plate
model. We also evaluated the resistance of biofilm-grown P. acnes towards
antimicrobial agents commonly used in the treatment of acne and the production
of putative virulence factors. Our results indicate that P. acnes can
form biofilms in vitro. The results also show that sessile P. acnes cells
are more resistant to various commonly used antimicrobial agents than
planktonic cells. In addition, sessile cells produce more extracellular
lipases as well as significant amounts of the quorum-sensing molecule
- PMID: 17399956 [PubMed - indexed for MEDLINE]