- J Gen Virol 84 (2003), 1021-1031; DOI 10.1099/vir.0.18788-0
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- Studies of the transmissibility of the agent of bovine
spongiform encephalopathy to pigs
- Gerald A. H. Wells1, Stephen A. C. Hawkins1, Anthony
R. Austin2, Stephen J. Ryder1, Stanley H. Done1, Robert B. Green1, Ian
Dexter1, Michael Dawson3 and Richard H. Kimberlin4
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- 1 Veterinary Laboratories Agency, Woodham Lane, New Haw,
Addlestone, Surrey KT15 3NB, UK
- 2 FARA, Oak Farm, Harpsden Bottom, Henley-on-Thames,
Oxon RG9 4HY, UK
- 3 NSPAC, DEFRA, Whittington Road, Worcester WR5 2SU,
UK
- 4 SARDAS, 27 Laverockdale Park, Edinburgh EH13 0QE, UK
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- Correspondence
- By Gerald Wells
- g.a.h.wells@vla.defra.gsi.gov.uk
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- Studies to test the transmissibility of the bovine spongiform
encephalopathy (BSE) agent to pigs began in 1989. Parenteral inoculation
of the agent by three routes simultaneously (intracranially, intravenously
and intraperitoneally) produced disease with an incubation period range
of 69150 weeks. Pre-clinical pathological changes were detected in two
pigs killed electively at 105 and 106 weeks post-inoculation. Infectivity
was detected by bioassay in inbred mice in the CNS of those pigs that developed
spongiform encephalopathy. Infectivity was also found in the stomach, jejunum,
distal ileum and pancreas of terminally affected pigs. These findings show
that pigs are susceptible to BSE. In contrast, disease failed to occur
in pigs retained for 7 years after exposure by feeding BSE-affected brain
on three separate days, at 12 week intervals. The amounts fed each day
were equivalent to the maximum daily intake of meat and bone meal in rations
for pigs aged 8 weeks. No infectivity was found in tissues assayed from
the pigs exposed orally. This included tissues of the alimentary tract.
It is suggested that these pigs did not become infected. The relatively
high oral exposure used in these experiments compared with feed-borne exposure
in the field may explain the absence of an epidemic of spongiform encephalopathy
in domestic pigs concurrent with the BSE epidemic in the UK
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- This article has been cited by other articles
-
-
- J. Castilla, A. Gutierrez-Adan, A. Brun, D. Doyle, B.
Pintado, M. A. Ramirez, F. J. Salguero, B. Parra, F. D. S. Segundo, J.
M. Sanchez-Vizcaino, M. Rogers, and J. M. Torres
- Subclinical Bovine Spongiform Encephalopathy Infection
in Transgenic Mice Expressing Porcine Prion Protein
- J. Neurosci., May 26, 2004; 24(21): 5063 - 5069.
- [Abstract] [Full Text] [PDF]
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- Patricia A. Doyle, PhD
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