- Hi Jeff -
-
- This was written up a year ago, January 2003. This means
that the work was done even before 2003, during 2002. More Proof...
-
- Patty
-
- Virology. 2003 Jan 5;305(1):192-200.
- Related Articles, Links
-
- Evaluation of a genetically modified reassortant H5N1
influenza A virus vaccine candidate generated by plasmid-based reverse
genetics.
-
- Subbarao K, Chen H, Swayne D, Mingay L, Fodor E, Brownlee
G, Xu X, Lu X, Katz J, Cox N, Matsuoka Y.
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- Influenza Branch, Centers for Disease Control and Prevention,
Atlanta, Georgia 30333, USA. KSubbarao@cdc.gov
-
- Avian influenza A H5N1 viruses similar to those that
infected humans in Hong Kong in 1997 continue to circulate in waterfowl
and have reemerged in poultry in the region, raising concerns that these
viruses could reappear in humans. The currently licensed trivalent inactivated
influenza vaccines contain hemagglutinin (HA) and neuraminidase genes from
epidemic strains in a background of internal genes derived from the vaccine
donor strain, A/Puerto Rico/8/34 (PR8). Such reassortant candidate vaccine
viruses are currently not licensed for the prevention of human infections
by H5N1 influenza viruses. A transfectant H5N1/PR8 virus was generated
by plasmid-based reverse genetics. The removal of the multibasic amino
acid motif in the HA gene associated with high pathogenicity in chickens,
and the new genotype of the H5N1/PR8 transfectant virus, attenuated the
virus for chickens and mice without altering the antigenicity of the HA.
A Formalin-inactivated vaccine prepared from this virus was immunogenic
and protected mice from subsequent wild-type H5N1 virus challenge. This
is the first successful attempt to develop an H5N1 vaccine seed virus resembling
those used in currently licensed influenza A vaccines with properties that
make it a promising candidate for further evaluation in humans.
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- PMID: 12504552 [PubMed - indexed for MEDLINE]
-
- Patricia A. Doyle, PhD
- Please visit my "Emerging Diseases" message
board at:
- http://www.clickitnews.com/ubbthreads/postlist.php?Cat=&Board=emergi
ngdiseases
- Zhan le Devlesa tai sastimasa
- Go with God and in Good Health
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