- Hello, Jeff - We did find that there were WNV+ mosquito
pools in Inperial County not far from the Salton Sea and now have found
21 sentinel chickens from 5 flocks have been found WNV+. Officially WNV
has gone border to border and coast to coast and into North America and
South America.
-
- WEST NILE VIRUS, SENTINEL CHICKENS - USA (CALIFORNIA)
-
- A ProMED-mail post http://www.promedmail.org ProMED-mail
is a program of the International Society for Infectious Diseases http://www.isid.org
-
- Source: California Arbovirus Surveillance Bulletin, Week
35, Thu 28 Aug 2003 [edited]
-
- http://westnile.ca.gov= /Arbovirus_Bulletin
-
-
- California: 5 Sentinel Chicken Flocks Confirmed West
Nile Virus-positive
-
- 21 chickens from 5 sentinel flocks in Imperial County
have been confirmed positive for West Nile virus (WNV) by the Viral and
Rickettsial Disease Laboratory (VRDL) and the Davis Arbovirus Research
Unit (DARU). The flocks are located near Seeley, El Centro, Niland, and
Holtville. Dried blood spots collected on 4 Aug 2003 from chickens in each
of these flocks screened positive for flavivirus (SLE/WNV). These results
were confirmed with blood collected last week.
-
- Dried blood spot specimens from sentinel chickens submitted
each week to the DHS Viral and Rickettsial Disease Laboratory (VRDL) are
tested for the presence of antibodies to western equine encephalomyelitis
(WEE), West Nile virus (WNV) and St. Louis encephalitis (SLE) viruses by
EIA and IFA. The VRDL forwards serum specimens from sentinel chicken in
which antibodies are detected in new geographic areas to the Davis Arbovirus
Research Unit (DARU) for additional testing for WNV, SLE, and WEE antibodies.
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- All mosquito pools are currently tested by in situ EIA
for WEE, SLE, and WNV. And all species except _Culex tarsalis_ are also
tested for California encephalitis virus. All pools are being tested by
Taqman for WNV (primers for WEE and SLE will soon be available to include
these viruses in the Taqman assay). Additional viral testing [may include
reverse transcriptase polymerase chain reaction (RT-PCR)] of mosquito pools
for California encephalitis and other arboviruses is performed as appropriate.
All dead bird kidney tissues are tested by DARU using the Taqman assay.
Organ pools from the carcasses are tested using cell culture for virus
isolation.
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- Eleanor Kellon, V.M.D. Drkellon@aol.com
-
- [Dr. Kellon has commented that one of these sites is
located in very close proximity to the Salton Sea (where a West Nile virus-positive
mosquito pool was reported previously); the others are located to the south
of this site. - Mod.CP]
-
-
- Patricia A. Doyle, PhD Please visit my "Emerging
Diseases" message board at: http://www.clickitnews.com/ubbthreads/postlist.php?Cat=&Board=emergingdiseases
Zhan le Devlesa tai sastimasa Go with God and in Good Health
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