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SARS - A Lab Accident?
From Dr. Robert Lee, Phd
rboblee@mchsi.com 5-25-3
5-25-3


Dear Jeff and Patty,
 
Information on palm civet cat is here: http://www.mns.org.my/article.php?sid=283
 
"Its feces are used in coffee made in Indonesia(What really happens is that the civet eats only the ripe coffee berries, and as the coffee bean itself is not digested, it is passed out. These are then collected, cleaned and dried...it is also supposed to be of high quality because the civet only selects ripe berries to consume)."
 
Note that coronavirus is shed in feces. If the palm civet is the source of SARS, then why has it not been widely spread in Indonesia consonant with the use of civet feces in making coffee there?
 
Whilst enjoying your morning coffee, read this... hopefully it is Columbian coffee and not Indonesian coffee...
 
If SARS is a civet cat disease that spread to humans (in some, as yet, unlikely manner) then this may be another vector:
 
More info on civet cat here: http://www.baobabfarm.com/index_data_en/Science/civetcat.htm
 
Order/family/genus/species of civet cats here: http://1kai.dokkyomed.ac.jp/mammal/en/order/carnivora.html
 
Diet of civet cat here: http://www.lioncrusher.com/animal.asp?animal=109 Diet includes "It will also eat birds, rodents and insects as well as fruit." Also note "Its feces are used in coffee made in Indonesia."
 
Feline coronaviruses are shed in feces... and they make coffee with this civet feces???
 
Birds and rodents have coronaviruses.
 
Phylogeny says the closest known relative to SARS E2-spike protein is an avian coronavirus but the ORF1a gene of SARS is almost exactly like murine hepatitis virus. I am skeptical of the civet cat as the orignal source of infection as cat coronaviruses will not naturally infect humans. The "toddy cat" (palm civet cat) is not a true cat but is in the order of carnivores which also includes dogs, bears, mongoose, "true" cats, and sea lions and seals. When the complete civet cat coronavirus is published, you can be sure that I will access it and compare it to SARS coronavirus.
 
Interesting articles on PubMed:
 
Vet Clin North Am Small Anim Pract 1993 Jan;23(1):1-16
 
Coronavirus infection in cats.
 
Hoskins JD.
 
Department of Veterinary Clinical Sciences, Louisiana State University School of Veterinary Medicine, Baton Route.
 
Cats are susceptible to natural infection with several strains of feline coronavirus that may result in either effusive and noneffusive FIP disease or in subclinical to severe enteritis. Investigators are still unsure of the routes by which strains of coronavirus are transmitted between cats. Excretion of coronavirus by infected cats into the environment occurs by way of feces, oronasal secretions, and, possibly, urine. FIP coronavirus remains stable outside the host for as long as 3 to 7 weeks and is rapidly inactivated by most household disinfectants. Clinical diagnosis of coronavirus infection is made by evaluating the presenting history, physical findings, laboratory results, coronavirus antibody titers, and by excluding analogous disease. The presence of coronavirus antibodies can be used to screen cats for the presence of coronavirus infection and as an adjunct in diagnosing clinical coronavirus infection. A intranasal TS-FIPV vaccine that protects against natural coronavirus challenge is available for healthy cats 16 weeks of age or older.
 
Publication Types: a.. Review b.. Review, Tutorial
 
PMID: 8380655
 
Vet Microbiol 1995 Feb;43(2-3):103-22
 
There is nothing permanent except change. The emergence of new virus diseases.
 
Truyen U, Parrish CR, Harder TC, Kaaden OR.
 
Institute for Medical Microbiology, Infectious and Epidemic Diseases, Ludwig Maximilians University, Munich, Germany.
 
The sudden appearance of apparently new viruses with pathogenic potential is of fundamental importance in medical microbiology and a constant threat to humans and animals. The emergence of a "new" pathogen is not an isolated event, as for instance the frequent appearance of new influenza virus strains demonstrates. Often the new virus strains co-circulate with the older strains in a susceptible population, but a replacement of the older strains has been also observed. In rare instances the new viruses can cause dramatic epidemics or pandemics, such as those observed with the human immunodeficiency virus, canine parvovirus, or most recently, with the agent of bovine spongiform encephalopathy in the United Kingdom. The mechanisms of the emergence are not always clearly understood, but an altered host range appears to be a common event. Whether a true change in host range occurs, or whether the virus adapted to the host and replicated more efficiently, is often unknown. This review tries to summarize the facts that are known about a wide variety of "new" viruses of mammals, such as the simian, human and feline lentiviruses, the feline coronaviruses, the feline parvoviruses, the carnivore morbilliviruses, the influenza A viruses, and the transmissible spongiform encephalopathies. A particular emphasis will be put on the genetic mechanisms that might have taken place and that might have been responsible for their sudden appearance.
 
Publication Types: a.. Review b.. Review, Tutorial
 
PMID: 7740750
 
 
 
Comment
 
Civet Cat Is Not A Feline
From Susan Mullen
s_mullen@spu.edu
5-28-3
 
I read the "SARS A Lab Virus?" post, and found several times the writer referred to the civet cat as a cat or feline. The civet cat, as shown by the definition below, is not a feline. As mentioned in the post, FIP is a very serious disease for cats. But any reference to felines just does not apply to civet cats.
 
Of the family Viverridae, the civet cat is a primarily nocturnal animal closely related to the mongoose. There are several species. Some are carnivores that live on the ground, while the animals with SARS in China are masked palm civets, which live in trees and eat fruit.

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